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PhD Fellowship (Molecular Neural Development and Sequencing Technologies) - University of Copenhagen

Project description

This project will use molecular tools including iTRAQ proteomics and single cell RNA-sequencing to characterize the progenitor stellate cell populations in the embryonic pig entorhinal cortex. In particular, characterisation of the progenitor cell populations that give rise to the stellate cells will be performed using bioinformatics. Further studies will be performed to derive stellate cells from induced pluripotent stem cells (iPSCs) using cytokines and growth factors identified from the global profiling and bioinformatics analyses. Comparative molecular studies (i.e. RNA-sequencing) will then be performed to assess whether stellate cells derived from Alzheimer disease patient iPSCs have an altered phenotype compared to iPSCs derived from healthy persons.

Required qualifications

  • Masters in Cell Biology, Neuroscience, Neural development, Bioinformatics or related field
  • Knowledge in pluripotent stem cell and/or developmental biology
  • Knowledge in neural development
  • Knowledge in cellular RNA and protein sequencing technologies
  • Experience in the field of neural development is desirable
  • Experience with proteomic sequencing and/or transcriptome sequencing is desirable
  • Experience with bioinformatics processing of large data sets is desirable
  • Experience with culture and differentiation of pluripotent stem cells is desirable
  • Experience with molecular methods such as immunohistochemistry, qPCR, westerns and cDNA library preparation is desirable
  • High motivation and basic scientific skills are essential
  • Excellent communication skills both oral and written, including fluent use of the English language
  • Excellent collaborating and teamwork skills are essential
  • Publication/s in relevant fields of the project is desirable

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